《植物生理学报》 2014, 50(6): 805-809

血叶兰的组织培养与快速繁殖

朱桥, 丁俊伟, 杨学文, 梁廉, 邵玲*

肇庆学院生命科学学院, 广东肇庆526061

通信作者：邵玲；E-mail: shaoling@zqu.edu.cn；Tel: 0758-2716359

摘　要：

以血叶兰的嫩茎段为外植体, 建立了血叶兰的组培快繁体系。结果表明: 芽诱导最适宜的培养基是MS+6-BA 5.0 mg•L^-1; 最适芽增殖培养基为MS+6-BA 5.0 mg•L^-1+NAA 0.5 mg•L^-1+TDZ 0.3 mg•L^-1, 芽增殖率达4.92倍; 最佳壮苗培养基为MS+NAA 0.3 mg•L^-1+GA₃ 1.0 mg•L^-1+15%椰汁, 芽苗高度达到4.33 cm, 芽粗为0.61 cm; 生根最适培养基为1/2MS+IBA 1.0 mg•L^-1+15%香蕉+0.5 g•L^-1活性炭, 生根率在93.0%以上; 炼苗后, 移栽在泥炭土+珍珠岩+松树皮(3:1:1, V/V/V)混合基质中, 存活率高于87.0%。

关键词：血叶兰; 组织培养; 快速繁殖

收稿：2014-03-20   修定：2014-04-22

资助：广东省大学生创新项目(1058012034)和肇庆市科技计划项目(2013N007)。

Tissue Culture and Rapid Propagation of Ludisia discolor

ZHU Qiao, DING Jun-Wei, YANG Xue-Wen, LIANG Lian, SHAO Ling*

College of Life Sciences, Zhaoqing University, Zhaoqing, Guangdong 526061, China

Corresponding author: SHAO Ling; E-mail: shaoling@zqu.edu.cn; Tel: 0758-2716359

Abstract:

This paper deals with tissue culture of Ludisia discolor by tender stem explants. The results showed the suitable medium of initial was MS medium supplemented with 5.0 mg•L^-1 6-BA. The best proliferation occurred on MS medium supplemented with 5.0 mg•L^-1 6-BA and 0.5 mg•L^-1 NAA and 0.3 mg•L^-1 TDZ, the propagation coefficient of adventitious shoots reached 4.92. The best hardening medium was MS medium supplemented with 0.3 mg•L-1 NAA and 1.0 mg•L^-1 GA₃ and 15% coconut milk, with the average plantlet height of 4.33 cm and the average stem diameter of 0.61 cm. The best rooting for plantlets occurred on 1/2MS medium supplemented with 1.0 mg•L^-1 IBA and 15% banana and 0.5 g•L^-1 activated carbons, with rooting rate up to 93.0%. The plantlets with acclimatization were transferred to the the mixed matrix [peat soil:vermiculite:pine bark=3:1:1 (V/V/V)] and the outplanting survival rate was 87.0%.

Key words: Ludisia discolor; tissue culture; rapid propagation